Platform and Pipeline

Vector Platform
CMC Platform
Manufacturing Platform

High Efficient Promotor Platform GritPAC^®

High efficient promotor and enhancer system is able to increase the protein expression largely. Gritgen's GritPAC^®platform has been demonstrated in non-human primate animal studies and clinical trials. GritPAC^® makes the microgram per liter of protein expression in blood into reality, that broadens our pipeline significantly.

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Next-generation AAV Capsid Platform GritOcul^®

Gritgen has developed a next-generation, propriatary AAV capsid platform GritOcul^®. Using the innovative capsid, gritgen is developing a long-lasting treatment for ophthamological disease.

AAV Process Development

1.Upstream process

01

HEK293 suspension
02

Three plasmids transfection
03

Lysis and harvest

2-4E14 vg/L

Upstream yield

2.Downstream process

01

Clarification
02

Column chromatography(affinity and anion exchange chromatography)
03

Ultrafiltration
04

Vertical filtration
05

Formulation

Plasmid Process Development

1.Upstream process

01

Cell thawing
02

Cell expansion in shake flask
03

Scale-up cultivation in fermenter
04

Cell harvest

＞85%

supercoiled ratio after fermentation

2.Downstream process

01

Continuous lysis
02

Column chromatography
03

Ultrafiltration

Quality Control and Release

Integrated quality control system, with full compliance on regulatory requirements and clarified release specifications, ensure the manufacturing capability to meet clinical and commercial demands.

Identification